Lepomis hybridization occurs frequently in natural systems. Previous investigations of sunfish hybridization have focused on courtship behavior of interspecific pairs. We observed spawning in Lepomis nesting colonies where interspecific nest intrusions occurred. Nesting male Lepomis microlophus (redear sunfish) attempted to fertilize eggs in the adjacent nests of spawning Lepomis megalotis (longear sunfish). Male sunfish commonly employ this reproductive tactic in the nests of conspecifics. When colonies contain more than one species, hybridization may occur through interspecific nest intrusion by males on adjacent nests.